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  1. The analysis of hydrophobic membrane proteins by two-dimensional gel electrophoresis has long been hampered by the concept of inherent difficulty due to solubility issues. We have optimized extraction protocol...

    Authors: Matthew A Churchward, R Hussain Butt, John C Lang, Kimberly K Hsu and Jens R Coorssen
    Citation: Proteome Science 2005 3:5
    Content type: Methodology Published on:

  2. A recombinant B. megaterium strain was used for the heterologous production of a glucosyltransferase (dextransucrase). To better understand the physiological and metabolic responses of the host cell to cultivatio...

    Authors: Wei Wang, Rajan Hollmann, Tobias Fürch, Manfred Nimtz, Marco Malten, Dieter Jahn and Wolf-Dieter Deckwer
    Citation: Proteome Science 2005 3:4
    Content type: Research Published on:

  4. Neuroglobin is a hexacoordinated member of the globin family of proteins. It is predominantly localized to various brain regions and retina where it may play a role in protection against ischemia and nitric ox...

    Authors: Begona Casado, Lewis K Pannell, Gail Whalen, Daniel J Clauw and James N Baraniuk
    Citation: Proteome Science 2005 3:2
    Content type: Research Published on:

  5. The structural integrity of recombinant proteins is of critical importance to their application as clinical treatments. Recombinant growth hormone preparations have been examined by several methodologies. In t...

    Authors: Felix Hepner, Edina Cszasar, Elisabeth Roitinger and Gert Lubec
    Citation: Proteome Science 2005 3:1
    Content type: Research Published on:

  6. Quantitative proteomics is an emerging field that encompasses multiplexed measurement of many known proteins in groups of experimental samples in order to identify differences between groups. Antibody arrays a...

    Authors: LT Perlee, J Christiansen, R Dondero, B Grimwade, S Lejnine, M Mullenix, W Shao, M Sorette, VT Tchernev, DD Patel and SF Kingsmore
    Citation: Proteome Science 2004 2:9
    Content type: Methodology Published on:

  7. Chaperones (CH) play an important role in tumor biology but no systematic work on expressional patterns has been reported so far. The aim of the study was therefore to present an analytical method for the conc...

    Authors: Jae-Kyung Myung, Leila Afjehi-Sadat, Maureen Felizardo-Cabatic, Irene Slavc and Gert Lubec
    Citation: Proteome Science 2004 2:8
    Content type: Research Published on:

  8. The aim of this study was firstly, to improve and validate a cerebrospinal fluid (CSF) prefractionation method followed by two-dimensional electrophoresis (2-DE) and secondly, using this strategy to investigat...

    Authors: Sara Folkesson Hansson, Maja Puchades, Kaj Blennow, Magnus Sjögren and Pia Davidsson
    Citation: Proteome Science 2004 2:7
    Content type: Research Published on:

  9. Quantitative proteomic analyses have traditionally used two-dimensional gel electrophoresis (2DE) for separation and characterisation of complex protein mixtures. Among the difficulties associated with this ap...

    Authors: Guennadi A Khoudoli, Iain M Porter, J Julian Blow and Jason R Swedlow
    Citation: Proteome Science 2004 2:6
    Content type: Methodology Published on:

  11. Structural genomics (SG) projects aim to determine thousands of protein structures by the development of high-throughput techniques for all steps of the experimental structure determination pipeline. Crucial t...

    Authors: Stéphane Raymond, Nicholas O'Toole and Miroslaw Cygler
    Citation: Proteome Science 2004 2:4
    Content type: Research Published on:

  13. Cu/Zn-superoxide dismutase 1 (SOD1), encoded on chromosome 21, is a key enzyme in the metabolism of reactive oxygen species (ROS) and pathogenetically relevant for several disease states including Down syndrom...

    Authors: Joo-Ho Shin, Jae-Won Yang, Marie Le Pecheur, Jacqueline London, Harald Hoeger and Gert Lubec
    Citation: Proteome Science 2004 2:2
    Content type: Research Published on:

  14. The rapid completion of genome sequences has created an infrastructure of biological information and provided essential information to link genes to gene products, proteins, the building blocks for cellular fu...

    Authors: Joo-Ho Shin, Jae-Won Yang, Jean-François Juranville, Michael Fountoulakis and Gert Lubec
    Citation: Proteome Science 2004 2:1
    Content type: Research Published on:

  15. Cerebral microdialysis has been established as a monitoring tool in neurocritically ill patients suffering from severe stroke. The technique allows to sample small molecules in the brain tissue for subsequent ...

    Authors: Martin H Maurer, Christian Berger, Margit Wolf, Carsten D Fütterer, Robert E Feldmann Jr, Stefan Schwab and Wolfgang Kuschinsky
    Citation: Proteome Science 2003 1:7
    Content type: Research Published on:

  16. Separation of proteins by two-dimensional gel electrophoresis (2-DE) coupled with identification of proteins through peptide mass fingerprinting (PMF) by matrix-assisted laser desorption ionization time-of-fli...

    Authors: Wei Wang, Jibin Sun, Manfred Nimtz, Wolf-Dieter Deckwer and An-Ping Zeng
    Citation: Proteome Science 2003 1:6
    Content type: Research Published on:

  17. Proteomics experiments typically involve protein or peptide separation steps coupled to the identification of many hundreds to thousands of peptides by mass spectrometry. Development of methodology and instrum...

    Authors: Gerard Cagney, Shiva Amiri, Thanuja Premawaradena, Micheal Lindo and Andrew Emili
    Citation: Proteome Science 2003 1:5
    Content type: Research Published on:

  18. Hippocampal neural stem cells (HNSC) play an important role in cerebral plasticity in the adult brain and may contribute to tissue repair in neurological disease. To describe their biological potential with re...

    Authors: Martin H Maurer, Robert E Feldmann Jr, Carsten D Fütterer and Wolfgang Kuschinsky
    Citation: Proteome Science 2003 1:4
    Content type: Research Published on:

  20. MALDI-TOF-MS has become an important analytical tool in the identification of proteins and evaluation of their role in biological processes. A typical protocol consists of sample purification, separation of pr...

    Authors: Florence Gonnet, Gilles Lemaître, Gilles Waksman and Jeanine Tortajada
    Citation: Proteome Science 2003 1:2
    Content type: Research Published on:
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Proteome Science

ISSN: 1477-5956