Fig. 5

Validation of differentially expressed proteins through Western blot analysis. The identified differential proteins were validated through Western blot in THP-1 macrophages. Validation was performed for WDR70, MYO1G, VAV1, SP1, CLTCL1, and TRIM24 proteins with actin as the reference protein (P-value < 0.05). THP-1 macrophages were infected intracellularly with Mycobacterium H37Ra at MOI = 10 for 24 h. NCTD was added 6 h post-infection and continued until 24 h to eliminate extracellular bacteria. Representative Western blots demonstrate the alteration in protein expression of MYO1G (A), WDR70 (B), CLTCL1 (C), VAV1 (D), SP1 (E), and TRIM24 (F) in the NCTD-treated group (5 μg/mL and 10 μg/mL) compared to the control group (DMSO and PBS). Data were obtained from three independent experiments and are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control