Fig. 7
From: NEK10 kinase ablation affects mitochondrial morphology, function and protein phosphorylation status
Analysis of total phosphorylation of threonine, serine, and tyrosine amino acids of candidate proteins. Immunoprecipitation of ATAD3, HSP60, and TOM20 from the crude mitochondrial fractionation of Parental and NEK10 KO cells (panels A-C). The lower part of the panels shows the total protein loading detected by the Bio-Rad Stain-Free Blot. The anti-rabbit antibody or anti-mouse IGG was used as the immunoprecipitation control. Anti-total threonine (D), and anti-total tyrosine (E) antibodies were used to detect phosphorylation by the western blotting of immunoprecipitates of ATAD3 (blue arrow), HSP60 (red arrow), and TOM20 (green arrow). Panel F shows the loading-normalized quantification of the blot from panel D. Panel G graphs the putative phosphorylation signals for these proteins from the LC–MS/MS analyses