Fig. 4
From: NEK10 kinase ablation affects mitochondrial morphology, function and protein phosphorylation status
Mitochondrial mass and superoxide analysis of Parental and NEK10 KO cells. A Histograms of flow cytometry analysis of cells treated or not with 0.1 µmol/l MitoBright Deep Red. B Histograms of flow cytometry analysis of cells treated or not with 20 nM MitoProbe TMRM. C Histograms of analysis of cells treated or not with MitoSox. In panel D cells were treated or not with 5 µM MitoSox + 20 µM Antimycin A (AA). The analysis was carried out on the BD FACSAria TM Ilu Special Order System and the histograms were analyzed on FCS Express 7 software. E The graphs show the quantification of mtDNA levels of Parental and NEK10 KO cells treated with 300 µg/ml zeocin or untreated. Total mtDNA levels were determined by comparing mtDNA ND1 (TET) or COX1 (FAM) with genomic DNA, actin (Cy5). The graphs show the result of four different passages. Statistical analyses were performed with GraphPad Prism, employing one-way ANOVA tests followed by Tukey's test, where p-value < 0.05 = *, p-value < 0.01 = **, and p-value < 0.001 ***. The graphs are shown in Mean with SD