Fig. 3
From: NEK10 kinase ablation affects mitochondrial morphology, function and protein phosphorylation status
Analysis of AIF and TOM20 protein expression of Parental and NEK10 KO cells by confocal microscopy. In panels, A and C show the Parental cells. Panels, B and D show the NEK10 KO cells. Panels A-B show AIF in green and the nuclear dye DAPI in blue. Panels C-D show TOM20 in green and the nuclear dye DAPI in blue In E and F, the graphs show the immunofluorescence intensity signal obtained by the Image J2 software [16]. Statistical analyses were performed with GraphPad Prism employing the unpaired t-test, where p-value < 0.05 = *, and p-value < 0.001 ***. The graphs show Mean ± SD. The images were obtained with the same intensity and brightness settings using a Leica Stellaris 5 confocal microscope