Fig. 2
From: NEK10 kinase ablation affects mitochondrial morphology, function and protein phosphorylation status
Morphological analysis of mitochondria in HAP1 Parental and NEK10 KO cells by Transmission Electron Microscopy (TEM). Panel A shows four representative micrographs of the mitochondria of the Parental cells; and panel B shows four representative pictures of the mitochondria of NEK10 KO cells. The green arrows mark examples of normal/elongated mitochondria, whereas the red arrows mark examples of fragmented/spherical mitochondria. In total, 14 micrographs were analyzed for Parental cells and 15 images for NEK10 KO cells. A total of 161 mitochondria from Parental cells and 291 mitochondria from NEK10 KO cells were analyzed. C graph showing the mitochondrial length (size in µm); D) mitochondrial width (width in µm); E) mitochondrial area (area in µm2); F) number of mitochondrial cristae per mitochondrion. The mitochondrion was classified based on their mitochondrial morphology: spherical (< 6 μm), or elongated (> = 6 μm) (panels G and H). p-value < 0.05 = *, p-value < 0.01 = **, p-value < 0.001 ***, and p-value < 0.0001 = ****. The graphs are shown in Mean ± SEM