Fig. 1
From: NEK10 kinase ablation affects mitochondrial morphology, function and protein phosphorylation status
Mitochondrial respiration of Parental and NEK10 KO cells. In A) The graph shows the OCR (Oxygen Consumption Rate) of Parental cells (in the blue line) and NEK10 KO cells (in the red line) by times in minutes. The black arrows show the time when the inhibitors were added with their respective concentration. B The graph shows the basal respiration of Parental and NEK10 KO cells. In C) Maximal OCR. D OCR related to proton leak. E ATP-linked OCR. F Spare Respiratory Capacity. Values were normalized by total proteins in µg/µl. The graphs show the results of 3 independent experiments (n = 3) in triplicate (3 different cell passages). The data was obtained with Seahorse XFp Analyzer – Agilent equipment and analyzed at Seahorse Wave desktop software. The statistical analyses were performed with GraphPad Prism and used the paired T-test * = p < 0.05. The graphs are shown in mean ± SD