Fig. 7

Assessment of the effect of panobinostat and hydroxychloroquine on LAMP2 protein expression. A and B. LAMP2 and β-actin were detected using western blotting using whole-cell proteins from U1 and U937 cells, respectively. C and D. Quantification of protein bands was performed using the ImageJ software. The cells were treated with panobinostat at concentrations of 0, 10, and 25 nM for 24 h (a concentration-dependent experiment), or 25 nM for 0, 24, and 48 h (a time-dependent experiment). Additionally, the cells were treated with hydroxychloroquine at concentrations of 0, 10, and 25 µM for 24 h (a concentration-dependent experiment) or 10 µM for 0, 24, and 48 h (a time-dependent experiment). Statistical analysis was performed using analysis of variance (ANOVA), and the p values are shown