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Fig. 2 | Proteome Science

Fig. 2

From: iTRAQ-based proteomic study on monocyte cell model discovered an association of LAMP2 downregulation with HIV-1 latency

Fig. 2

Plasma membrane (PM) enrichment efficacy detected using western blotting A. Detection of PM enrichment efficacy in U1 and U937 cells using western blotting. B. Quantification of protein bands using ImageJ software. Na+/K+-ATPase serves as a biomarker of PM, prohibitin as a biomarker of mitochondrion, and β-actin as a reference protein. Hom, TM and PM represent the homogenate of whole-cell, total membrane, and plasma membrane, respectively. The PM was enriched by more than 5- and 2-fold in the PM and TM fractions, compared with the homogenates in both U1 and U937 cells. *, **, ***, **** represent statistical significance levels of p < 0.05, 0.01, 0.005 and 0.001, respectively. The experiments were replicated triple

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Proteome Science

ISSN: 1477-5956