Fig. 3

Validation of the cAMP signaling pathway-related proteomics results using PCR and western blotting. A Schematic representation of the cAMP signaling pathway; 7 upregulated genes are highlighted in red. Heatmap showing age-related changes in the expression levels of the seven genes; B RT-PCR results of the selected three genes from the altered seven genes (n = 4). C Western blotting of phosphorylated PKA and total PKA (n = 4)