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Fig. 1 | Proteome Science

Fig. 1

From: Identification of different proteins binding to Na, K-ATPase α1 in LPS-induced ARDS cell model by proteomic analysis

Fig. 1

The workflow of AP-MS (IP-MS) technology. Using this approach with no IP-level antibodies are available against the target protein. Target protein(bait) is co-expressed with affinity tag and forms a complex with the endogenous components, then purified with immobilized tag affinity protein dynabeads and identified with LC–MS/MS after extensively washing off unspecifically bound proteins

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Proteome Science

ISSN: 1477-5956