Fig. 2

2-DE profiles (pH 4–7) of protein samples from medaka. Proteins were extracted using a Trizol method, b lysis buffer method, c TCA/acetone precipitation, d aliquot Trizol method and e. Trizol method with a commercial clean-up kit. A total of 100 μL of each protein extract was loaded onto the strips and silver staining was used. Magnified images of selected areas on the 2-DE profile of each method are shown at the right bottom corner