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Figure 9 | Proteome Science

Figure 9

From: Promoter trapping method: transcription factor purification using human telomerase reverse transcriptase promoter

Figure 9

Replicate experiments using PT from cell lines HEK293 and HeLa cell. Protein identification of PT elute was achieved with the use of mass spectrometry and analyzed with Scaffold version 3.6.2, Proteome Software Inc. Any protein identified with greater than a 99% possibility and two unique peptides with at least 95% probability were considered. Reproducibility of triplicate PT experiments with HEK293 cell line is shown by the overlapping region of HEK293 PT 1, 2, and 3 with 208 proteins in common. HeLa cell line was also compared in duplicate and was found to have 86 proteins which occur in both experiments. 60 proteins were found to be in common in all five of the experiments. These proteins are specified in an Excel spreadsheet file in Additional file 2: Table S2.

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Proteome Science

ISSN: 1477-5956