Figure 5

Affinity purification procedure is accompanied by removal and enhancement of different proteins. Fold changes were calculated from the normalized and corrected sum of the peptides measured for selected proteins and protein families in Paca44 affinity pure and crude EV samples. The numbers of peptides measured in each sample were corrected and normalized as described in particular in the Materials and Methods. Proteins, e.g. tetraspanins and CD proteins, are enriched, whereas laminins and proteasome proteins were removed.