Figure 2

Affinity purifications of EV samples: a) Using the anchor proteins of EVs TACSTD1/Epcam or MFGE8 the exosomal marker proteins as CD9, SDCBP/Syntenin and CD63 were enriched in the eluates (El) after affinity purifications of EVs whereas no or smaller amount of exosomal proteins were detected in flow through (FT). Samples were eluted with PBS or NP40. b) Immunoblots confirmed that proteins like proteasome subunit alpha and tubulin beta partially remain in the flow through. c) Treatments of affinity purified samples with detergents (Triton × 100) allowed the passage of exosomal marker proteins through the ultrafilter membrane (cut off at 100 kDa). Ret, retentate; Fil filtrate.