Figure 6

ANXA2 translocates from the membrane/organelle fraction to the nucleus in response to prednisone. Shown is immunoblot analysis of C2C12 subcellular fractions probed for ANXA2 (A), and quantitation of experimental triplicates densitometry (B). ANXA2 was not detected in the cytoskeleton fraction (data not shown). ANXA2 migrated from the membrane/organelle fraction to the nucleus within 15 minutes of prednisone exposure (**= p ≤ 0.05). Data in Panel B are mean ± SE.