Figure 4

Correlation of protein abundances estimated by APEX vs . 2-DE based on protein M r . Proteins quantitated by APEX and 2-DE were compared against all proteins predicted for the Sd197 genome sorted by Mr in bins of 10 kDa width (Fig. 4a). Correlation of protein abundances estimated by APEX vs. 2-DE decreased for low Mr proteins at <20 kDa (R2= 0.51), but increased for proteins in the 20 – 70 kDa Mr range (R2= 0.73) compared to the overall abundance correlation (R2= 0.67).