Figure 5

2-DE spots that yielded different MS/MS identifications in polymer fractions than in a total protein extract. 2-DE gel pattern of UPP peak 1 proteins (pink) was overlaid with gel pattern of UPP peak 2 proteins (green). Spots labeled with red numbers were identified as LMW-GS in the UPP peak 1 fraction but either gliadins, triticin or different LMW-GS in a total protein fraction. In at least one of the four fractions, spots labeled with underscored numbers contained chain-terminating gliadins as the predominant proteins.